Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-Regimes

Exploring the natural genetic variability and its exploitation for improved Nitrogen Use Efficiency (NUE) in sorghum is one of primary goals modern crop improvement programs. The integrated strategies include high-throughput phenotyping, next generation sequencing (NGS)-based genotyping technologies, a priori selected candidate gene studies that help understand detailed physiological molecular mechanisms underpinning this complex trait. A set sixty diverse genotypes was evaluated different vegetative, reproductive, yield traits related to NUE field (under three N regimes) two seasons. Significant variations under 0 50% confirmed availability native low regimes. Sorghum with distinct background had interestingly similar associated traits. Genotyping-By-Sequencing based SNPs (>89 K) were used study population structure, phylogenetic groupings identified groups. information grain stalk content individuals covered on groups indicated randomness distribution adaptation variable This promising consistent performance varying environments, buffer capacity conditions. We also report better performing varied production use—grain, stover, dual-purpose having differential response Expression profiling genes shoot root tissues contrasting lines (PVK801 HDW703) grown conditions revealed interesting outcomes. Root exhibited expression profiles transporter [ammonium ( SbAMT) , nitrate transporters SbNRT )]; assimilatory (glutamine synthetase SbGS) glutamate synthase SbGOGAT[NADH], SbGOGAT[Fd]) [nitrite reductase SbNiR[NADH]3 amino acid biosynthesis [glutamate dehydrogenase SbGDH )]. Identification dosages will provide new toward regimes sorghum. High from could be potential candidates in-depth analysis contribute development efficient cultivars.